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KMID : 0525619980030030268
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1998 Volume.3 No. 3 p.268 ~ p.278
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Commercial Production and Applications of Chitosanase
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최연진/Choi YJ
김은정/김영수/신용철/김종진/윤영철/최치만/Kim EJ/Kim YS/Shin YC/Kim JJ/Yoon YC/Choi CM
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Abstract
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Fermentation conditions and purification processes for chitosanase production from Bacillus sp. KCTC0337BP were optimized using 50 liter jar fermentor. Maximum 70U/ml of chitosanase was produced from the bacterium under optimized conditions. From the culture supernatant, chitosanase was purified 28-fold by a series of purification steps containing membrane filtration, ultrafiltration, and ion exchange chromatography. The specific activity and recovery yield of the purified enzyme was 1,009U/mg and 68%, respectivety. Chitosanase activity of lyophilized enzyme was 200,000U/g for desalted enzyme and 45,000U/g for non-desalted enzyme. The purified chitosanase was characterized and its applicability for chitin and chitosan oligomer productions was examined. Our results indicated that the enzyme can be applied to commercial production of chitosan oligomer with high degree of polymerization, low viscosity or water-soluble chitosans, and chitin oligomer from partially deacetylated chitin.
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